Third stage of downstage processing in production of API
In this stage of downstage processing we have to purify the liquid substance. This stage is called concentration. Concentration: In this stage of downstream processing we apply some techniques to purify the liquid. The substance has more than 80% of liquid that we have to purify. After the removal of liquid we achieve our actual […]
Different chromatography techniques as used in downstream processing
Higher value products demand for chromatographic techniques. The techniques are used for concentration, de salting and purification of protein preparation. When choosing a chromatographic technique, a number of factors need to be put into consideration. These factors may include iso- electric point, biological affinity, hydro-phobicity and molecular weight in proteins. Each of the above properties […]
Release of intracellular components in downstream processing
Targets like recombinant proteins and many enzymes are intracellular in downstream processes. Sometimes breaching of cytoplasm membrane and the cell wall can bring problems. This can occur when the cell has a strong cell wall. The liberation of the DNA which can, in the long run increase viscosity of suspension is some of the many […]