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Third stage of downstage processing in production of API

Posted by admin on October 30, 2016 in Uncategorized |
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In this stage of downstage processing we have to purify the liquid substance. This stage is called concentration. Concentration: In this stage of downstream processing we apply some techniques to purify the liquid. The substance has more than 80% of liquid that we have to purify. After the removal of liquid we achieve our actual […]

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Different chromatography techniques as used in downstream processing

Posted by admin on October 13, 2016 in Uncategorized |
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Higher value products demand for chromatographic techniques. The techniques are used for concentration, de salting and purification of protein preparation. When choosing a chromatographic technique, a number of factors need to be put into consideration. These factors may include iso- electric point, biological affinity, hydro-phobicity and molecular weight in proteins. Each of the above properties […]

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Release of intracellular components in downstream processing

Posted by admin on October 2, 2016 in Uncategorized |
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Targets like recombinant proteins and many enzymes are intracellular in downstream processes. Sometimes breaching of cytoplasm membrane and the cell wall can bring problems. This can occur when the cell has a strong cell wall. The liberation of the DNA which can, in the long run increase viscosity of suspension is some of the many […]

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Downstream Processing – Stage I

Posted by admin on August 29, 2016 in Uncategorized |
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Solid-Liquid Separation The first step or stage of the downstream processing is called Solid-Liquid Separation. Solid-Liquid Separation includes the breaking of the cells and elements that are insoluble from the fermentation broth. If the task is to produce an intracellular metabolite, then the intracellular metabolite has to be released from the cells before the Solid-Liquid […]

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